Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Naïve CD8+T cells were isolated by FACS, followed by activation with anti-CD3 and CD28 antibody for 18hr in vitro. RNA was extracted using Trizol reagent. 1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols