Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted from fresh frozen tumor tissues using AllPrep DNA/RNA/Protein Kit (Qiagen). RNA library was prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA) and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs).