Instrument: BGISEQ-500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Lungs were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. High-throughput sequencing was performed at the BGISEQ platform. Qualified RNA samples were digested with DNA I enzyme and then poly (A) containing mRNA was purified using Oligo (dT) containing beads. Next, mRNA was fragmented and reverse transcribed to first- and second-strand. Double-strand DNA ends were repaired and modified. Then DNA was amplified via PCR and the products were thermally denatured into single-stranded and cyclized. After rolling circle amplification and quality control, high-throughput sequencing was performed at the BGISEQ platform.