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SRX12162752: GSM5576140: S4; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 76M spots, 7G bases, 2.1Gb downloads

Submitted by: NCBI (GEO)
Study: RNAseq anaysis of hippocamal parvalbumin-positive interneurons in mice exposed to chronic social defat stress
show Abstracthide Abstract
Purpose: To identify genes differentially expressed in parvalbumin-positive (PV) interneurons of the hippocampus in non-defetaed (CTRL), stress-susceptible and stress-resilient mice. Method: Translating Ribosome Affinity Purification (TRAP) to isolate RNA from PV+ cells, cDNA synthesis and next generation RNAseq using Illumina Nextseq 500 sequencer. Results: RNA-seq revealed 458 DEGs between non-defeated and resilient mice, 1976 DEGs in non-defeated and susceptible mice, and 3475 DEGs in resilient and susceptible mice. Overall design: Cre-dependent EGFP-L10a mice crossed with PV-cre mice (EGFP-L10a+; PV-Cre+) were exposed to chronic social defeat stress. Two hippocampi from one mouse were pooled for one TRAP sample. CTRL is a TRAP/RNAseq dataset for non-defeat group. TRL Input is RNAseq dataset for the total RNA sample of non-defeat group before TRAP (translating ribosome affinity purification) procedure.
Sample: S4
SAMN21415682 • SRS10141081 • All experiments • All runs
Organism: Mus musculus
Instrument: NextSeq 500
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: TRAP RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM5576140
Runs: 1 run, 76M spots, 7G bases, 2.1Gb
Run# of Spots# of BasesSizePublished


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