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SRX12195249: GSM5580101: pEXO_macrophage_01; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 28.9M spots, 8.7G bases, 2.4Gb downloads

Submitted by: NCBI (GEO)
Study: Placental exosomes polarize human monocyte-derived macrophages to a decidual M2-like macrophage phenotype
show Abstracthide Abstract
Macrophages are abundant in uterine mucosa during the peri-implantation phase and early pregnancy. Decidual macrophages display dynamic changes alone with pregnancy progress: During the peri-implantation phase, macrophages displayed a pro-inflammatory phenotype which facilitates embryo implantation. While, In the late firster trimester and second trimester, decidual macrophages are anti-proinflammatory which are helpful to pregnancy maintenance. Alterations in the ratio of pro-inflammatory/anti-inflammatory decidual macrophages lead to abortion, preeclampsia, and preterm birth. Placenta-derived exosomes (pEXO) are critical in the immune cell modulation such as T cell apoptosis, NK activities, and T regulatory (Treg) differentiation. However, it is unknown whether placenta-derived exosomes contribute to decidual macrophage polarization during early pregnancy. Here we report that exosomes from the placenta explant stimulate M2 macrophage polarization via exosomal miRNA-30d-5p. Mechanistically, miRNA-30d-5p polarized macrophages to M2 phenotype by inhibiting HDAC9 expression. Furthermore, the conditioned medium of pEXO-treated macrophages promoted trophoblast migration and invasion. By contrast, conditioned medium impaired the ability of endothelial cell tube formation. However, pEXO-treated macrophages have no impact on T cell proliferation. Together, we demonstrated that pEXO promoted trophoblast migration and invasion, endothelial cell migration, and attenuation of endothelial cell tube formation by polarizing macrophage to decidua-like macrophage. Overall design: mRNA profiles of pEXO-treated macrophages (N=2) and Control macrophage (N=2)
Sample: pEXO_macrophage_01
SAMN21442171 • SRS10172688 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted by RNA spin column The libraries were prepared according to Illumina TruSeq® RNA sample preparation Kit.
Experiment attributes:
GEO Accession: GSM5580101
Links:
Runs: 1 run, 28.9M spots, 8.7G bases, 2.4Gb
Run# of Spots# of BasesSizePublished
SRR1590469028,902,8308.7G2.4Gb2021-09-18

ID:
16115022

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