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SRX12261276: GSM5589191: Mock-IR rep 2; Mus musculus; RNA-Seq
2 ILLUMINA (NextSeq 500) runs: 27.7M spots, 2.1G bases, 710.5Mb downloads

Submitted by: NCBI (GEO)
Study: Elimination of radiation-induced senescence in the brain tumor microenvironment attenuates glioblastoma recurrence
show Abstracthide Abstract
We report gene expression changes in the brains of C57BL6/J mice 30 days after cranial irradiation with 10 Gy of X-rays Overall design: Examination of RNA profiles of brains of C57BL6/J mice at 30 days post irradiation compared to mock-irradiated mice (3 mice per group)
Sample: Mock-IR rep 2
SAMN21522508 • SRS10236403 • All experiments • All runs
Organism: Mus musculus
Instrument: NextSeq 500
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: At 30 days post-IR, mice were perfused with PBS and brains were dissected out. Total RNA was extracted from dissected whole brains using TRIzol (Invitrogen) according to the manufacturer's instructions. RNA quantity was then determined using a Qubit fluorometer (Thermo Fisher), and RNA quality was analyzed with an RNA tape on the 4200 TapeStation (Agilent). TruSeq Stranded Total RNA Ribo-Zero kit (Illumina) was used to prepare sequencing libraries from 2 μg total RNA. Sequencing was performed on the NextSeq500 using 75 nucleotide single end SBS chemistry, with ~30‐40 Million reads for each sample.
Experiment attributes:
GEO Accession: GSM5589191
Runs: 2 runs, 27.7M spots, 2.1G bases, 710.5Mb
Run# of Spots# of BasesSizePublished


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