GSM5592484: Fed_GAA 2; Mus musculus; RNA-Seq - SRA

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SRX12286484: GSM5592484: Fed_GAA 2; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 550) run: 11.6M spots, 1.8G bases, 641.8Mb downloads

Submitted by: NCBI (GEO)

Study: Regulation and role of glycophagy in skeletal muscle energy metabolism

PRJNA765027 • SRP338102 • All experiments • All runs

show Abstracthide Abstract

We report that short-term knockdown of the enzyme alpha-acid glucosidase in mouse C2C12 myotubes modifies genes involved in PPAR and PI3K-AKT signaling. Overall design: C2C12 myoblasts were transfected with shscrambled or shGAA lentivirus, puromycin selected, and then differentiated into myotubes which were then harvested for RNA sequencing.

Sample: Fed_GAA 2

SAMN21541001 • SRS10260376 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 550

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was extracted using a QIAGEN Rneasy minikit (Cat # 74004) and 1 ug of total RNA was used for the construction of sequencing libraries RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM5592484

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 11.6M spots, 1.8G bases, 641.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR15998566	11,618,420	1.8G	641.8Mb	2021-09-24

ID:: 16206257

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