EXPERIMENT_TYPE: mRNA-Seq
EXTRACTION_PROTOCOL: Ambion mirVana miRNA isolation kit
EXTRACTION_PROTOCOL_MRNA_ENRICHMENT:
Part of Library Prep, Truseq RNA-Seq Sample Prep K (show full text...)(hide...)Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
RNA_PREPARATION_INITIAL_RNA_QNTY: 1ug
RNA_PREPARATION_REVERSE_TRANSCRIPTION_PRIMER_SEQUENCE: Random Primers(hexamers)
RNA_PREPARATION_REVERSE_TRANSCRIPTION_PROTOCOL: TruSeq RNA
LIBRARY_GENERATION_PCR_TEMPLATE: cDNA
LIBRARY_FRAGMENTATION:
Part of Library Prep, Truseq RNA-Seq Sample Prep K (show full text...)(hide...)Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
LIBRARY_FRAGMENT_SIZE_RANGE: 120-200bp
LIBRARY_GENERATION_PCR_POLYMERASE_TYPE: Proprietary
LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM:
98C 30 sec, 15 cycles of 98C 10 sec, 65C 30 sec, 7 (show full text...)(hide...)98C 30 sec, 15 cycles of 98C 10 sec, 65C 30 sec, 72C 30 sec, then 72C 5 min, 4C hold
LIBRARY_GENERATION_PCR_NUMBER_CYCLES: 15
LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE: Proprietary
LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE: Proprietary
LIBRARY_GENERATION_PCR_PRIMER_CONC: Proprietary
LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL:
Part of Library Prep, Truseq RNA-Seq Sample Prep K (show full text...)(hide...)Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
GEO Accession: GSM1220585