amplicon-seq of human gene editing site : PBMCs - SRA

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SRX15405362: amplicon-seq of human gene editing site : PBMCs
1 ILLUMINA (Illumina HiSeq X) run: 41,205 spots, 12.4M bases, 4.8Mb downloads

Design: Genomic DNA was extracted from PBMCs of two patient donors and a healthy donor. Specifically, target loci were amplified with corresponding primers first. After another round of PCR with primers containing adapter, amplicons were sequenced for 2x300 paired-end reads with the Illumina Sequencing System.

Submitted by: East China Normal University

Study: First 2 TDT patientis gene editing outcomes

PRJNA839164 • SRP376010 • All experiments • All runs

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This is a non-randomized, open label, single-dose, phase 1/2 study in up to 12 participants with beta-thalassemia major.This study aims to evaluate the safety and efficacy of the treatment with gama-globin reactivated autologous hematopoietic stem cells in subjects with beta-thalassemia major. The next-generation sequencing data deposited here includes editing results in patient cells, as well as patient PBMC single-cell transcriptomes.

Sample:

SAMN28512362 • SRS13076566 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: amp151

Instrument: Illumina HiSeq X

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 41,205 spots, 12.4M bases, 4.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR19346254	41,205	12.4M	4.8Mb	2022-05-22

ID:: 21947236

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