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Design: Myeloid-biased HSC require Semaphorin 4A from the bone marrow niche for self-renewal under stress and life-long persistence
Submitted by: Helmholtz Munich
Study: Myeloid-biased HSC require Semaphorin 4A from the bone marrow niche for self-renewal under stress and life-long persistence
show Abstracthide Abstract
Tissue stem cells are hierarchically organized, but the signals that selectively preserve their functional integrity are largely unknown. Semaphorin 4A (Sema4A) is a specific regulator of myeloid-biased hematopoietic stem cells (myHSC), which are positioned at the top of the HSC hierarchy. Through single-cell transcriptomics, we compare the effect of Sema4A knock-out on the myHSCs.
Library:
Name: SLX-19432.i729_i516.HK3H3BBXY.s_2_s
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC SINGLE CELL
Selection: RT-PCR
Layout: SINGLE
Construction protocol: Bone marrow mononuclear cells were stained with conjugated monoclonal antibodies for HSPC markers as described above except for CD34 in order to minimize staining time and prevent RNA degradation (CD34 staining requires 90 minutes (Okamoto et al., 2007). Single cells were sorted into 96-well plates with the lysis buffer and stored frozen at -80. cDNA amplification and sequencing were performed as per Smart-Seq2 protocol (Picelli et al., 2013). Multiplexed sequencing libraries were prepared from cDNA using the Illumina Nextera XT protocol.
Experiment attributes: (show all 4 attributes...) (hide...)
Experimental Factor: inferred cell type: hematopoietic stem cell
Experimental Factor: single cell identifier: SLX-19432.i729_i516.HK3H3BBXY.s_2.r_1
Experimental Factor: protocol: Smart-seq2
Experimental Factor: genotype: wild type genotype
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ID:
21947092

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