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ERX9299447: Illumina NovaSeq 6000 paired end sequencing; Myeloid-biased HSC require Semaphorin 4A from the bone marrow niche for self-renewal under stress and life-long persistence
2 ILLUMINA (Illumina NovaSeq 6000) runs: 298.3M spots, 37.3G bases, 10.7Gb downloads

Design: Myeloid-biased HSC require Semaphorin 4A from the bone marrow niche for self-renewal under stress and life-long persistence
Submitted by: Helmholtz Munich
Study: Myeloid-biased HSC require Semaphorin 4A from the bone marrow niche for self-renewal under stress and life-long persistence
PRJEB52992 • ERP137746 • All experiments • All runs
show Abstracthide Abstract
Tissue stem cells are hierarchically organized, but the signals that selectively preserve their functional integrity are largely unknown. Semaphorin 4A (Sema4A) is a specific regulator of myeloid-biased hematopoietic stem cells (myHSC), which are positioned at the top of the HSC hierarchy. Through single-cell transcriptomics, we compare the effect of Sema4A knock-out on the myHSCs.
Sample: WT2_S3
SAMEA14418513 • ERS12024075 • All experiments • All runs
Organism: Mus musculus
Library:
Name: WT2_S3_p
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC SINGLE CELL
Selection: RT-PCR
Layout: PAIRED
Construction protocol: BMMNC were stained with conjugated monoclonal antibodies for HSPC markers as described above. 10,000 Lin-kit+ cells from young or aged WT/Sema4AKO mice were sorted in Ca2+/Mg2+-free phosphate-buffered saline (D-PBS) supplemented with 2% bovine serum albumin (New England Biolabs). Following one round of centrifugation, the cells were processed on 10x Genomics platform according to the manufacturer's protocol. Single Cell 3′ Reagent Kit v3 (10x Genomics) according to the manufacturer's protocol. Libraries from 10,000 Kit+ cells each from WT (n=1) and Sema4A KO (n=2) young and WT (n=2) old mice were prepared using the Single Cell 3′ Reagent Kit v3 (10x Genomics) according to the manufacturer's protocol.
Experiment attributes:
Experimental Factor: inferred cell type: myeloid-biased hematopoietic stem cell
Experimental Factor: protocol: 10xv3
Experimental Factor: genotype: wild type genotype
Runs: 2 runs, 298.3M spots, 37.3G bases, 10.7Gb
Run# of Spots# of BasesSizePublished
ERR9751600149,388,11818.7G5.4Gb2023-09-27
ERR9751599148,940,00518.6G5.3Gb2023-09-27

ID:
21947098

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