Name: GSM6432360
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: CjiPSCs cultured in the presence or absence of feeders, in the presence or absence of IWR1, were collected. To minimize the contamination of feeder cells, >30 colonies of cjiPSCs cultured on feeder layer colonies were randomly picked up under inverted microscope and pooled before isolation of total RNA. Total RNA was extracted by RNeasy Plus Micro Kit (#74034, QIAGEN). RNA-seq libraries were made by SMRT-Seq HT plus kit (#R400748, Takara) by following the protocol. Briefly, total RNA was quantified by Qubit and RNA integrity was checked by Tapstation. Then 1 ng RNA was used for cDNA conversion following one-step first-strand cDNA synthesis and double-stranded cDNA amplification protocol. cDNA was purified by AMPxp beads, concentration was measued by Qubit, and quality was checked by Tapstation. Next, 2 ng cDNA was used for library construction. Libraries were dual indexed and pooled by equal molecular concentration. 100-base pair reads were sequenced on Illumina Hiseq 2000.