HTS of MARV passaged isolate - SRA

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SRX21227679: HTS of MARV passaged isolate
1 ILLUMINA (Illumina MiSeq) run: 10,671 spots, 1.7M bases, 773,743b downloads

Design: Viral RNA was extracted using a Qiagen's QIAmp Viral RNA Mini Kit or RNeasy Mini Kit. The purified RNA served as template to generate cDNA using the SuperScript IV First-Strand Synthesis System (Invitrogen), and was subsequently turned into double-stranded DNA with the NEBNEXT mRNA Second Strand Synthesis Module (New England Biolabs). Target enrichment using in-solution hybridization capture (myBaits Hybridization Capture for Targeted NGS protocol ) was used for deep-sequencing.

Submitted by: Robert Koch institute

Study: Filovirus sequencing

PRJNA1001398 • SRP453002 • All experiments • All runs

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Sequences of in vitro and in vivo passaging of filoviruses. The goal is to evaluate virus adaptation to different hosts.

Sample:

SAMN36810534 • SRS18483400 • All experiments • All runs

Organism: Orthomarburgvirus marburgense

Library:

Name: DM3

Instrument: Illumina MiSeq

Strategy: WGS

Source: VIRAL RNA

Selection: PCR

Layout: SINGLE

Runs: 1 run, 10,671 spots, 1.7M bases, 773,743b

Run	# of Spots	# of Bases	Size	Published
SRR25496580	10,671	1.7M	773,743b	2023-09-29

ID:: 28664304

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