Tiled RT-PCR of SARS-CoV-2 from wastewater. - SRA

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SRX24466857: Tiled RT-PCR of SARS-CoV-2 from wastewater.
1 ILLUMINA (NextSeq 2000) run: 3.3M spots, 1.7G bases, 486.8Mb downloads

Design: Total nucleic acid from Ceres Nanotrap concentrate of wastewater influent treated with TurboDNase, concentrated, reverse transcribed with LunaScript, amplified with ARTIC v5.3.2 primers using NEB Q5, converted into Illumina-compatible libraries using NEB Ultra II DNA kit, and sequenced on an Illumina system 250PE or 300PE.

Submitted by: Verily Life Sciences LLC (verily_ww)

Study: SARS-CoV-2 wastewater surveillance (Verily Life Sciences/NWSS)

PRJNA1027353 • SRP473785 • All experiments • All runs

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Raw sequencing data targeting SARS-CoV-2 in wastewater samples. These data were generated from wastewater samples tested by Verily Life Sciences for monitoring SARS-CoV-2 lineages in wastewater for CDC NWSS.

Sample:

SAMN41232928 • SRS21218732 • All experiments • All runs

Organism: wastewater metagenome

Library:

Name: VLT_1897-240425.1

Instrument: NextSeq 2000

Strategy: AMPLICON

Source: METAGENOMIC

Selection: RT-PCR

Layout: PAIRED

Runs: 1 run, 3.3M spots, 1.7G bases, 486.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR28910499	3,265,806	1.7G	486.8Mb	2024-05-06

ID:: 32783034

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