Wikel isolate 6 - SRA

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SRX1392456: Wikel isolate 6
1 ILLUMINA (Illumina HiSeq 2500) run: 6.5M spots, 904.1M bases, 673.9Mb downloads

Design: Genomic DNA from each individual was digested with Sbf I-HF restriction enzyme was ligated to the unique P1 RAD adapter primers. Adapter ligated DNA fragments from individual samples were pooled and sonicated. DNA fragments between 400-600 bp were extracted using 1% agarose gel. Blunt ends were repaired and A-overhangs were then added using Klenow fragment (3’-5’ exo). P2 RAD adapter was then ligated to the DNA.

Submitted by: Purdue University

Study: Ixodes scapularis genome sequencing

PRJNA299499 • SRP065406 • All experiments • All runs

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Illumina RADseq project to evaluate the genetic structure and population genomics of the black-legged tick, Ixodes scapularis (the tick vector of Lyme disease, babesiosis, human granulocytic anaplasmosis and Powassan virus) across North America.

Sample: Ixodes scapularis Wikel Strain (UTMB Colony) Female 1

SAMN04215265 • SRS1137815 • All experiments • All runs

Organism: Ixodes scapularis

Library:

Name: WK6

Instrument: Illumina HiSeq 2500

Strategy: WGS

Source: GENOMIC

Selection: Restriction Digest

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 6.5M spots, 904.1M bases, 673.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR2845331	6,457,683	904.1M	673.9Mb	2015-12-15

ID:: 1978215

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