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SRX1716907: SIV sequencing of plasma from cy0382 at necropsy by Illumina
1 ILLUMINA (Illumina MiSeq) run: 866,288 spots, 203M bases, 74.9Mb downloads

Design: This involved isolating vRNA, generating cDNA with the SSIII RT-PCR kit and sequence specific primers, fragmenting the cDNA with the Nextera XT kit, tagging the library pieces, and then sequencing on the Illumina MiSeq.
Submitted by: UW-Madison
Study: Simian immunodeficiency virus Genome sequencing and assembly
show Abstracthide Abstract
These sequences refer to those described in our manuscript, "Vaccination with Live Attenuated Simian Immunodeficiency Virus (SIV) Protects from Mucosal, but not Necessarily Intravenous, Challenge with a Minimally Heterologous SIV". Here, there were three animals who had been immunized with a live attenuated SIV and then challenged with a pathogenic SIV containing different sequences. These three animals developed high viremia after this challenge. We sequenced the replicating virus to determine if it was a recombinant of the live attenuated SIV and the challenge virus.
Sample: SIV sample from cy0382 at necropsy
SAMN04625824 • SRS1405774 • All experiments • All runs
Library:
Name: cy0382_SIV_Necropsy
Instrument: Illumina MiSeq
Strategy: OTHER
Source: VIRAL RNA
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 866,288 spots, 203M bases, 74.9Mb
Run# of Spots# of BasesSizePublished
SRR3407247866,288203M74.9Mb2016-04-26

ID:
2460400

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