The Positive Transcriptional Elongation Factor (P-TEFb) is required f... - SRA

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SRX1834645: The Positive Transcriptional Elongation Factor (P-TEFb) is required for Neural Crest Specification
1 ILLUMINA (Illumina HiSeq 2000) run: 47.1M spots, 9.4G bases, 5.5Gb downloads

Design: For RNA sequencing total RNA was fragmented before undergoing cDNA library preparation. cDNA was end-repaired, A-tailed and adapter-ligated before amplification and size selection. The prepared libraries undergo quality control by running on a gel and using a bioanalyser to check library fragment size distribution before 100bp paired end sequencing over one lane of a flow cell. Samples were multiplexed 2 x 3 and ran on one lane each to yield approximately 25 million paired reads per sample.

Submitted by: TGAC

Study: Xenopus laevis RNA-Seq on animal caps

PRJNA325078 • SRP076316 • All experiments • All runs

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RNA-Seq on Xenopus laevis animal caps induced to form neural crest, neuroectoderm or ectoderm in the presence and absence of leflunomide.

Sample: Stage 8 animal caps were injected with Noggin and Wnt to induce neural crest and fixed and sequenced at stage 15.

SAMN05219200 • SRS1494144 • All experiments • All runs

Organism: Xenopus laevis laevis

Library:

Name: WTCHG_37663_221

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 47.1M spots, 9.4G bases, 5.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR3654127	47,088,345	9.4G	5.5Gb	2016-06-10

ID:: 2619178

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