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SRX1834646: The Positive Transcriptional Elongation Factor (P-TEFb) is required for Neural Crest Specification
1 ILLUMINA (Illumina HiSeq 2000) run: 46.5M spots, 9.3G bases, 5.4Gb downloads

Design: library preparation. cDNA was end-repaired, A-tailed and adapter-ligated before amplification and size selection. The prepared libraries undergo quality control by running on a gel and using a bioanalyser to check library fragment size distribution before 100bp paired end sequencing over one lane of a flow cell. Samples were multiplexed 2 x 3 and ran on one lane each to yield approximately 25 million paired reads per sample.
Submitted by: TGAC
Study: Xenopus laevis RNA-Seq on animal caps
show Abstracthide Abstract
RNA-Seq on Xenopus laevis animal caps induced to form neural crest, neuroectoderm or ectoderm in the presence and absence of leflunomide.
Sample: Stage 8 animal caps were injected with Noggin and Wnt to induce neural crest, treated with leflunomide and fixed and sequenced at stage 15.
SAMN05219202 • SRS1494145 • All experiments • All runs
Library:
Name: WTCHG_37663_222
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: PAIRED
Spot descriptor:
forward101  reverse

Runs: 1 run, 46.5M spots, 9.3G bases, 5.4Gb
Run# of Spots# of BasesSizePublished
SRR365414246,466,5159.3G5.4Gb2016-06-10

ID:
2619179

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