RNA-Seq of Spodoptera litura: midgut of 5th instar larvea feeding on ... - SRA

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SRX2174005: RNA-Seq of Spodoptera litura: midgut of 5th instar larvea feeding on Brassica juncea for 6h
1 ILLUMINA (Illumina HiSeq 2000) run: 11.3M spots, 1.1G bases, 733.6Mb downloads

Design: Firstly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads.Then mRNA was break into short pieces with fragmentation buffer. First strand cDNA was synthesized using random hexamer primer. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. The overhangs of DNA fragments were converted into blunt ends. After adenylation of 3ĄŻ ends of DNA fragments, sequence adaptor was added. 150~200 bp length cDNA fragments were purified with AMPure XP beads. Then PCR was performed and products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.

Submitted by: South China Normal University

Study: Spodoptera litura Transcriptome or Gene expression

PRJNA283976 • SRP058373 • All experiments • All runs

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Transcriptome of Spodoptera litura midgut and head

Sample: Generic sample from Spodoptera litura

SAMN05751539 • SRS1701506 • All experiments • All runs

Organism: Spodoptera litura

Library:

Name: Mg_6h

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RT-PCR

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 11.3M spots, 1.1G bases, 733.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR4254091	11,273,525	1.1G	733.6Mb	2017-10-01

ID:: 3168931

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