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SRX2463137: RNA-seq of Nicotiana benthamiana Mock-innoculated roots 18 hai
1 ILLUMINA (Illumina HiSeq 2500) run: 60.5M spots, 12.2G bases, 7.4Gb downloads

Design: N. benthamiana mRNAs were purified using Poly(A) selection from total RNA sample and then fragmented. cDNA library preparation was performed with the TruSeq® RNA Sample Preparation Kit (Illumina US) according to the manufacturer’s protocol.
Submitted by: Sainsbury Laboratory (SLCU), University of Cambridge
Study: Dual transcriptome of Nicotiana benthamiana infected with Phytophthora palmivora
show Abstracthide Abstract
Phytophthora palmivora is the most prevalent oomycete plant pathogen species in the tropics. It causes significant economic losses in numerous unrelated monocot and dicot crops. Next generation plant resistance breeding relies on knowledge of the pathogens repertoire of pathogen secreted proteins, however genetic resources for this species are limited. Studying the dynamic transcriptome of P. palmivora in plant model system interactions provides valuable information on its infection strategies. Here we take dual transcriptomics approach, performing deep time-resolved RNA-seq of N. benthamiana infected with P. palmivora, followed by de novo assembly of P. palmivora transcriptome. This gives us an advantage to track transcriptional dynamics in both interacting organism simultaneously.
Sample: Host plant mock-inoculated
SAMN06116083 • SRS1895699 • All experiments • All runs
Library:
Name: NB_Mock_18hai
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RT-PCR
Layout: PAIRED
Runs: 1 run, 60.5M spots, 12.2G bases, 7.4Gb
Run# of Spots# of BasesSizePublished
SRR514467960,544,74912.2G7.4Gb2017-01-09

ID:
3576090

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