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SRX2463139: RNA-seq of Phytophthora palmivora axenically grown mycelium
1 ILLUMINA (Illumina Genome Analyzer II) run: 32.2M spots, 4.9G bases, 2.9Gb downloads

Design: mRNAs were purified using Poly(A) selection from total RNA sample. cDNA libraries were prepared using NEBNext® RNA library preparation kit (New England Biolabs UK) according to the manufacturer’s protocol and sequenced on Illumina GAII Genome Analyzer in a 76 paired end mode
Submitted by: Sainsbury Laboratory (SLCU), University of Cambridge
Study: Dual transcriptome of Nicotiana benthamiana infected with Phytophthora palmivora
show Abstracthide Abstract
Phytophthora palmivora is the most prevalent oomycete plant pathogen species in the tropics. It causes significant economic losses in numerous unrelated monocot and dicot crops. Next generation plant resistance breeding relies on knowledge of the pathogens repertoire of pathogen secreted proteins, however genetic resources for this species are limited. Studying the dynamic transcriptome of P. palmivora in plant model system interactions provides valuable information on its infection strategies. Here we take dual transcriptomics approach, performing deep time-resolved RNA-seq of N. benthamiana infected with P. palmivora, followed by de novo assembly of P. palmivora transcriptome. This gives us an advantage to track transcriptional dynamics in both interacting organism simultaneously.
Sample: P. palmivora axenically grown mycelium
SAMN06116944 • SRS1895701 • All experiments • All runs
Library:
Name: PP_myc
Instrument: Illumina Genome Analyzer II
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RT-PCR
Layout: PAIRED
Runs: 1 run, 32.2M spots, 4.9G bases, 2.9Gb
Run# of Spots# of BasesSizePublished
SRR514468132,151,7584.9G2.9Gb2017-01-09

ID:
3576092

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