GSM3133386: CON rep1; Mus musculus; RNA-Seq - SRA

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SRX4048942: GSM3133386: CON rep1; Mus musculus; RNA-Seq
1 ION_TORRENT (Ion Torrent Proton) run: 44.1M spots, 5.2G bases, 3.8Gb downloads

Submitted by: NCBI (GEO)

Study: RNA-sequencing analysis of aberrantly expressed long non-coding RNAs and mRNAs in a mouse model of ventilator-induced lung injury

PRJNA464384 • SRP144777 • All experiments • All runs

show Abstracthide Abstract

Long non-coding RNAs are closely associated with the regulation of various biological processes and are involved in the pathogenesis of numerous diseases. However, the role of lncRNAs in ventilator-induced lung injury (VILI) has yet to be evaluated. This study aims to evaluate the expression profile and role of lncRNAs and mRNAs in VILI. Overall design: In the present study, high-throughput sequencing was applied to investigate differentially expressed lncRNAs and mRNAs (fold change >2; false discovery rate <0.05). Bioinformatics analysis was employed to predict the functions of differentially expressed lncRNAs.

Sample: CON rep1

SAMN09083358 • SRS3266058 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Ion Torrent Proton

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction. Ion Proton Sequencing Protocol

Experiment attributes:

GEO Accession: GSM3133386

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 44.1M spots, 5.2G bases, 3.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR7127940	44,120,282	5.2G	3.8Gb	2021-05-08

ID:: 5526898

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