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SRX4510343: GSM3319349: DGRP-229_Infected; Drosophila melanogaster; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 26.6M spots, 2.7G bases, 1.7Gb downloads

Submitted by: NCBI (GEO)
Study: RNA-sequencing of the female adult fly guts from 38 DGRP lines with and without oral infection with Pseudomonas entomophila
show Abstracthide Abstract
In order to gain insights into gut immunocompetence and its variation in a population, we performed RNA-sequencing of whole guts of 38 Drosophila Genetic Reference Panel strains. Overall design: 76 gut samples profiled from 38 DGRP strains under two conditions: control and infected with Pseudomonas entomophila.
Sample: DGRP-229_Infected
SAMN09768084 • SRS3629753 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: For each condition, guts from 30 adult female flies were collected in PBS four hours post treatment and directly homogenized in trizol (Invitrogen) using a bead-beater. A standard protocol for RNA extraction was performed. Illumina stranded Truseq libraries
Experiment attributes:
GEO Accession: GSM3319349
Links:
Runs: 1 run, 26.6M spots, 2.7G bases, 1.7Gb
Run# of Spots# of BasesSizePublished
SRR764739626,643,0052.7G1.7Gb2019-10-07

ID:
6108009

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