Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was extracted from the samples with a Maxwell® 16 Total RNA Purification Kit. Library preparation was done with a poly(A) selection kit (KAPA mRNA HyperPrep) to enrich for mRNAs. RNA libraries were prepared for sequencing using standard Illumina protocols. Multiplexed paired end sequencing (PE76) was done using an Illumina NextSeq500 on multiple flow cells. We blocked for lane batch effects by splitting the quadruplet samples into pairs and ran two pairs of each set per flow cell.