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SRX8356236: GSM4557341: PBS_SD; Mus musculus; RNA-Seq
4 ILLUMINA (Illumina HiSeq 4000) runs: 80.3M spots, 10.2G bases, 5Gb downloads

Submitted by: NCBI (GEO)
Study: Diet-induced obesity promotes infection by impairment of the innate antimicrobial defense function of dermal adipocyte progenitors
show Abstracthide Abstract
We report the transcriptomes of single cells isolated from whole skin of wild type B6 male mice fed a standard or 60% high fat diet 48-hours after mock- or S. aureus-infection. Overall design: Wild type B6 male mice fed a standard or 60% high fat diet ad libitum were mock- or S. aureus-infected. Two days post-infection whole skin samples were collected and processed for 3'-end single cell RNA-sequencing.
Sample: PBS_SD
SAMN14943471 • SRS6672441 • All experiments • All runs
Organism: Mus musculus
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Single cells were isolated as previously described (Guerrero-Juarez, et al. Nat Commun. 2019) Single cells were captured using the Chromium Platform (10X Genomics) and libraries were generated using Single Cell 3' v3 chemistry. Cell capture, library preparation, quality control, and sequencing was performed at the UCI Genomics High Throughput Facility at the University of California, Irvine. Single Cell 3' v3 chemistry - 10X Genomics
Experiment attributes:
GEO Accession: GSM4557341
Runs: 4 runs, 80.3M spots, 10.2G bases, 5Gb
Run# of Spots# of BasesSizePublished


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