WGS of KSHV BAC16 Delete KapC - SRA

SRX11122242: WGS of KSHV BAC16 Delete KapC
1 ILLUMINA (Illumina MiSeq) run: 348,591 spots, 167.7M bases, 85.4Mb downloads

Design: DNA was extracting using Qiagen Large Construct Kit. Libraries were prepared using a NEB ultra II DNA Library Prep Kit for Illumina and sequenced using a MiSeq 500 v2 nano run.

Submitted by: University of Calgary

Study: Kaposi's sarcoma-associated herpesvirus BAC16 kaposin-deficient viruses

PRJNA736974 • SRP323750 • All experiments • All runs

show Abstracthide Abstract

The goal of this study overall was to create a panel of KSHV viruses targeting the polycistronic kaposin locus for deletion to enable characterization of individual protein function.The goal of this sequencing was to determine if recombination during mutagenesis had occurred as expected and that no off-target mutations arose.

Sample:

SAMN19672118 • SRS9185510 • All experiments • All runs

Organism: Human gammaherpesvirus 8

Library:

Name: KSHVdelC

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM PCR

Layout: PAIRED

Runs: 1 run, 348,591 spots, 167.7M bases, 85.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR14789027	348,591	167.7M	85.4Mb	2021-06-11

ID:: 14806614

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