RNA-seq of mus musculus: adult bone marrow stromal cells - SRA

SRX12204833: RNA-seq of mus musculus: adult bone marrow stromal cells
1 DNBSEQ (DNBSEQ-G400) run: 48.8M spots, 4.9G bases, 2.8Gb downloads

Design: Cell suspension from digested leg bones were pooled together with flushed BM fraction after CD45+ leukocyte depletion. The RNA was extracted using RNeasy Mini Kit (QIAGEN) and DNA was removed using RNase-Free DNase Set (QIAGEN). RNA-seq was performed by BGI Genomics (Hong Kong). Library selection: poly-A selection method.

Submitted by: Children's Hospital of Philadelphia

Study: RNA-seq to analyze how Sbds deletion affects BM niche gene expression at baseline and following TBI

PRJNA763892 • SRP337441 • All experiments • All runs

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To define mechanisms by which SBDS deficiency in bone marrow (BM) niches impairs donor hematopoietic stem cell engraftment after transplantation, we performed RNA-seq on the BM stromal cells from Sbds-deficient mice versus control mice at 24 hours after myeloablative total body irradiation (TBI) and at baseline.

Sample: SBDS Baseline

SAMN21461625 • SRS10181133 • All experiments • All runs

Organism: Mus musculus

Library:

Name: HK500MUSuqtEAADRAAPEI-595

Instrument: DNBSEQ-G400

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: other

Layout: PAIRED

Runs: 1 run, 48.8M spots, 4.9G bases, 2.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR15914599	48,796,070	4.9G	2.8Gb	2021-09-16

ID:: 16124606

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