ChIP-Seq Analysis of H3K27me3 in hESC H1 Cells - SRA

SRX007379: ChIP-Seq Analysis of H3K27me3 in hESC H1 Cells
1 ILLUMINA (Illumina Genome Analyzer II) run: 12.7M spots, 963.8M bases, 4.6Gb downloads

Design: Single read - Illumina

Submitted by: Gene Expression Omnibus (GEO)

Study: BI Human Reference Epigenome Mapping Project

• SRP000996 • All experiments • All runs

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The NIH Roadmap Epigenomics Mapping Consortium aims to produce a public resource of epigenomic maps for stem cells and primary ex vivo tissues selected to represent the normal counterparts of tissues and organ systems frequently involved in human disease. Characterization of the reference epigenome in humans by use of ChIP-Seq in a diverse panel of ES cells, tissue stem cells, reprogrammed stem cells, primary cells and tissues.

Sample: Generic sample from Homo sapiens

SAMN00002925 • SRS004524 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: Solexa-8039

Instrument: Illumina Genome Analyzer II

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Spot descriptor:

1 forward

Experiment attributes: (show all 14 attributes...) (hide...)

EXPERIMENT_TYPE: Histone H3K27me3

EXTRACTION_PROTOCOL: SDS lysis

EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Bioruptor

EXTRACTION_PROTOCOL_SONICATION_CYCLES: 1

CHIP_PROTOCOL: standard

CHIP_PROTOCOL_CHROMATIN_AMOUNT: standard

CHIP_PROTOCOL_BEAD_TYPE: standard

CHIP_PROTOCOL_BEAD_AMOUNT: standard

CHIP_PROTOCOL_ANTIBODY_AMOUNT: standard

CHIP_ANTIBODY: H3K27Me3

CHIP_ANTIBODY_PROVIDER: Upstate

CHIP_ANTIBODY_CATALOG: 07-449

CHIP_ANTIBODY_LOT: DAM1387952

GEO accession: GSM433167

Links:

External link: EDACC Genboree Page

External link: GEO Web Link

Runs: 1 run, 12.7M spots, 963.8M bases, 4.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR020508	12,682,151	963.8M	4.6Gb	2009-08-21

ID:: 7645

SRA

Sequence Read Archive

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