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SRX020952: GSM544720: 3T3-L1_0hr_CEBPb
1 ILLUMINA (Illumina Genome Analyzer II) run: 15.2M spots, 548.5M bases, 369.9Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Propagation of Adipogenic Signals through an Epigenomic Transition State
show Abstracthide Abstract
The transcriptional mechanisms by which temporary exposure to developmental signals instigates adipocyte differentiation are unknown. During early adipogenesis, we find transient enrichment of the glucocorticoid receptor (GR), CCAAT/enhancer binding protein b (CEBPb), p300, mediator subunit 1, and histone H3 acetylation near genes involved in cell proliferation, development and differentiation, including the gene encoding the master regulator of adipocyte differentiation, peroxisome proliferator activated receptor g2 (PPARg2). Occupancy and enhancer function are triggered by adipogenic signals, and diminish upon their removal. GR, which is required for adipogenesis but need not be active in the mature adipocyte, transiently functions with other enhancer proteins to propagate a new program of gene expression that includes induction of PPARg2, thereby providing a memory of the earlier adipogenic signal. Thus, the conversion of preadipocyte to adipocytes involves the formation of an epigenomic transition state that is not observed in cells at the beginning or end of the differentiation process. Overall design: Genomic occupancy profiled by high throughput sequencing (ChIP-seq) from 3T3-L1 cells during differentiation for H3K9Ac, CEBPb and GR.
Sample: 3T3-L1_0hr_CEBPb
SAMN00013668 • SRS073118 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM544720: 3T3-L1_0hr_CEBPb
Instrument: Illumina Genome Analyzer II
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Spot descriptor:
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Experiment attributes:
GEO Accession: GSM544720
Links:
External link:
Runs: 1 run, 15.2M spots, 548.5M bases, 369.9Mb
Run# of Spots# of BasesSizePublished
SRR04924615,237,492548.5M369.9Mb2010-06-04

ID:
22468

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