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SRX029266: GSM610747: G9a_f_f_4h_pIC_H3K9me2_redo
1 ILLUMINA (Illumina Genome Analyzer II) run: 41.1M spots, 1.6G bases, 883Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response (sequencing)
show Abstracthide Abstract
Effective anti-viral immunity depends on the ability of infected cells or cells triggered with virus-derived nucleic acids to produce type I interferon (IFN), which activates transcription of numerous antiviral genes. However, disproportionately strong or chronic IFN expression is a common cause of inflammatory and autoimmune diseases. Here we describe an epigenetic mechanism that determines cell-type specific differences in IFN and IFN-stimulated gene expression in response to exogenous signals. We identify di-methylation of histone H3 at lysine 9 (H3K9me2) as a suppressor of IFN and IFN-inducible antiviral gene expression. We show that levels of H3K9me2 at IFN and IFN stimulated genes (ISG) correlate inversely with the scope and amplitude of IFN and ISG expression in fibroblasts and dendritic cells. Accordingly, genetic ablation or pharmacological inactivation of lysine methyltransferase G9a, which is essential for the generation of H3K9me2, resulted in phenotypic conversion of fibroblasts into highly potent IFN-producing cells and rendered these cells resistant to pathogenic RNA viruses. In summary, our studies implicate H3K9me2 and enzymes controlling its abundance as key regulators of innate antiviral immunity. Overall design: Examination of epigenetic modifications in WT and G9a deficient MEFs and splenic DCs
Sample: G9a_f_f_4h_pIC_H3K9me2_redo
SAMN00116674 • SRS118207 • All experiments • All runs
Organism: Mus musculus
Name: GSM610747: G9a_f_f_4h_pIC_H3K9me2_redo
Instrument: Illumina Genome Analyzer II
Strategy: ChIP-Seq
Selection: ChIP
Layout: SINGLE
Spot descriptor:

Experiment attributes:
GEO Accession: GSM610747
External link:
Runs: 1 run, 41.1M spots, 1.6G bases, 883Mb
Run# of Spots# of BasesSizePublished


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