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SRX11358759: GSM5419304: 18hr KO CD8+T cells rep1; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 28M spots, 8.4G bases, 2.4Gb downloads

Submitted by: NCBI (GEO)
Study: Brd4 regulates the homeostasis of CD8+ T-lymphocytes and their proliferation in response to antigen stimulation [RNA-seq]
show Abstracthide Abstract
Purpose: The goals of this study are to compare the differentially expressed genes between in wild type and Brd4-/- CD8+T cells. Methods: RNA extracted from wild type and Brd4-/- CD8+T cells was used to generate RNA-seq library,followed by sequencing. Results: In naïve CD8+T cells, Brd4 deletion casued 956 downregulated genes and 841 upregulated genes. In in vitro-activated CD8+T cells(18hr),Brd4 deletion resulted in 1182 downregulated genes and 789 upregulated genes. Importantly,we noticed that Brd4 is required for the migration- and glucose-related genes. expression Conclusions: Our study represents the first detailed analysis of transcriptomes in wild type and Brd4-/- CD8+T cells. Our results show that Brd4 deletion resulted in more downregulated genes exceeding the upregulated genes and the downregulation of many migration- and glucose-ralated genes. We conclude that Brd4-mediated gene expresssion program promotes glucose metabolism and cell migartion. Overall design: mRNA profiles of wild type (WT) and Brd4-/- CD8+T cells
Sample: 18hr KO CD8+T cells rep1
SAMN20066127 • SRS9405232 • All experiments • All runs
Organism: Mus musculus
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Naïve CD8+T cells were isolated by FACS, followed by activation with anti-CD3 and CD28 antibody for 18hr in vitro. RNA was extracted using Trizol reagent. 1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM5419304
Runs: 1 run, 28M spots, 8.4G bases, 2.4Gb
Run# of Spots# of BasesSizePublished


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