Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Mesenchymal cells were isolated from fresh lung by negative sorting of Cd45, Cd31, Cd326. Cellular suspensions were loaded on a Chromium Controller instrument (10x Genomics) to generate single-cell Gel-Bead-in-Emulsions (GEMs) and perform single cell partitioning and barcoding. 10x Genomics Single Cell 3' RNA-seq kit v3 User Guide