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SRX12155387: GSM5575921: CM-5; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 58.6M spots, 3G bases, 970.7Mb downloads

Submitted by: NCBI (GEO)
Study: Targeting miR-126 in inv(16) acute myeloid leukemia inhibits leukemia development and leukemia stem cell maintenance
show Abstracthide Abstract
Acute myeloid leukemia (AML) harboring inv(16)(p13q22) expresses high levels of miR-126. Here we show that the CBFB-MYH11 (CM) fusion gene upregulates miR-126 expression through aberrant miR-126 transcription and perturbed miR-126 biogenesis via the HDAC8/RAN-XPO5-RCC1 axis. Aberrant miR-126 upregulation promotes survival of leukemia-initiating progenitors and is critical for initiating and maintaining CM-driven AML. We show that miR-126 enhances MYC activity through the SPRED1/PLK2-ERK-MYC axis. Notably, genetic deletion of miR-126 significantly reduced AML rate and extended survival in CM knock-in mice. Therapeutic depletion of miR-126 with an anti-miR-126 (miRisten) inhibited AML cell survival, reduced leukemia burden and leukemia stem cell (LSC) activity in inv(16) AML murine and xenograft models. Combination of miRisten with chemotherapy further enhanced the anti-leukemia and anti-LSC activity. Overall, this study provides molecular insights for the mechanism and impact of miR-126 dysregulation in leukemogenesis and highlights the potential of miR-126 depletion as a new therapeutic approach for inv(16) AML. Overall design: Total 10 LSK samples were analyzed in this study. 5 samples as CM, 5 samples as CM/miR-126?/?
Sample: CM-5
SAMN21412395 • SRS10133728 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Library contruction was used KAPA RNA HyperPrep Kit with RiboErase (HMR) according to manufacturer's protocol. Depletion of ribosomal RNA (rRNA) and the rapid construction of stranded RNA-Seq libraries
Experiment attributes:
GEO Accession: GSM5575921
Links:
Runs: 1 run, 58.6M spots, 3G bases, 970.7Mb
Run# of Spots# of BasesSizePublished
SRR1586433658,617,6453G970.7Mb2021-09-16

ID:
16075156

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