GSM5628782: Control E4.5 Embryo replicate 9; Mus musculus; RNA-Seq - SRA

SRX12633793: GSM5628782: Control E4.5 Embryo replicate 9; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 27.4M spots, 8.3G bases, 2.6Gb downloads

Submitted by: NCBI (GEO)

Study: Klf5 confers embryonic and extra-embryonic bi-potential cell fate in early blastomeres and embryonic stem cells

PRJNA771686 • SRP341620 • All experiments • All runs

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This study examines the transcriptomes of embryonic stem cells following overexpression of Klf5. Additionally, this study examines the transcripomes of single embryos either treated with scramble siRNA or siRNA targeting Klf5. Overall design: Bulk RNA-seq was performed on mESCs overexpressing Klf5 or GFP as a control. Single embryo RNA-seq was performed on single embryos injected with either a scramble control siRNA, or an siRNA specific to Klf5

Sample: Control E4.5 Embryo replicate 9

SAMN22329297 • SRS10587171 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA extraction and cDNA synthesis was performed using SMARTseq-V4 kit. cDNA was then purified with AMPure XP beads RNA-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645), diluting NEB adaptors 1:5 and performing 8 PCR cycles.

Experiment attributes:

GEO Accession: GSM5628782

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 27.4M spots, 8.3G bases, 2.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR16356317	27,397,813	8.3G	2.6Gb	2021-10-21

ID:: 17175797

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