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SRX12633798: GSM5628787: Klf5 Knockdown E4.5 Embryo replicate 4; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 24.8M spots, 7.5G bases, 2.3Gb downloads

Submitted by: NCBI (GEO)
Study: Klf5 confers embryonic and extra-embryonic bi-potential cell fate in early blastomeres and embryonic stem cells
show Abstracthide Abstract
This study examines the transcriptomes of embryonic stem cells following overexpression of Klf5. Additionally, this study examines the transcripomes of single embryos either treated with scramble siRNA or siRNA targeting Klf5. Overall design: Bulk RNA-seq was performed on mESCs overexpressing Klf5 or GFP as a control. Single embryo RNA-seq was performed on single embryos injected with either a scramble control siRNA, or an siRNA specific to Klf5
Sample: Klf5 Knockdown E4.5 Embryo replicate 4
SAMN22329302 • SRS10587176 • All experiments • All runs
Organism: Mus musculus
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA extraction and cDNA synthesis was performed using SMARTseq-V4 kit. cDNA was then purified with AMPure XP beads RNA-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645), diluting NEB adaptors 1:5 and performing 8 PCR cycles.
Experiment attributes:
GEO Accession: GSM5628787
Runs: 1 run, 24.8M spots, 7.5G bases, 2.3Gb
Run# of Spots# of BasesSizePublished


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