Name: GSM6042121
Instrument: Illumina HiSeq 4000
Strategy: Bisulfite-Seq
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Construction protocol: Zygotes were collected from 6-8-week-old female mice after hormone simulation and mating. The zygotes were cultured in vitro to collect embryos at different developmental stages.The embryos were washed in PBS and transferred into acidic Tyrode's solution to remove zonae pellucidae. Single zona-free embryos were finally lysed and processed following the scNMT-seq protocol. Genomic DNA in the supernatant after capturing of poly-A RNA of each sample was processed following scNMT-Seq protocol to construct the single embryo bisulfite sequencing library.