Instrument: Illumina HiSeq 2500
Strategy: ncRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: smallRNA libraries were assembled using the Truseq small RNA kit (Illumima) according to the maunfacturers instructions. smallRNAs of 22-10nt in length were selected for sequencing. 6x10e6 sorted cells were then centrifuged and RNA immediately extracted using the Qiagen miRNAeasy mini kit (Qiagen, UK) according to the manufacturer’s instructions. Quality and purity of the extracted total RNA was verified using spectrophotometry and the Agilent RNA 6000 nano kit according to manufacturer’s instructions