Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA isolated from cells using the SMARTer Ultra Low RNA Kit for the Fluidigm C1 System (Clontech). Library construction: In accordance to protocol from Fluidigm describing the use of the C1 system to generate cDNA library for single cell RNA-seq. Briefly, cDNA was synthesized using SMARTer Ultra Low RNA Kit for the Fluidigm C1 System from Clontech; library was constructed using Nextera XT DNA Sample Preparation Kit and Nextera XT DNA Library Preparation Index Kits from Illumina.