Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Cells were washed and harvested using Qiagen RNeasy mini kit. 10ug of RNA was DNase treated and cleaned up with Rneasy MinElute Cleanup Kit. Total stranded RNA libraries were construced with a standard dUTP protocol. For a the drug screen the previously published Tag-Seq protocol was followed.