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SRX2578600: GSM2496164: H3K27ac ChIP-seq Primary Epidermal Keratinocyte Biological Replicate 2 - Differentiation Day 3 - ZNF750 siRNA; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 35.1M spots, 3.5G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)
Study: Dynamic and stable enhancer-promoter contacts regulate terminal differentiation [ChIP-Seq]
PRJNA330779 • SRP079141 • All experiments • All runs
show Abstracthide Abstract
Genome-wide chromosome conformation capture (Hi-C) and promoter-capture Hi-C (CHi-C) were performed during epidermal differentiation. These data indicate that dynamic and constitutive enhancer-promoter contacts combine to control gene induction during differentiation and that chromosome conformation enables discovery of new TFs with distinct roles in this process. Overall design: ChIP-seq data from epidermal keratinocyte progenitor and differentiated cells.
Sample: H3K27ac ChIP-seq Primary Epidermal Keratinocyte Biological Replicate 2 - Differentiation Day 3 - ZNF750 siRNA
SAMN06350015 • SRS1993189 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 4000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Cells were crosslinked with 1% formaldehyde for 10 minutes, then quenched with 0.125M glycine for 5 minutes. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with an antibody. NEB Next ChIP-seq library prep kit was used.
Experiment attributes:
GEO Accession: GSM2496164
Links:
Runs: 1 run, 35.1M spots, 3.5G bases, 1.3Gb
Run# of Spots# of BasesSizePublished
SRR527461635,125,5343.5G1.3Gb2017-06-30

ID:
3720992

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