GSM2576294: RNA-Seq AMPK-KO rep2; Mus musculus; RNA-Seq - SRA

SRX2734510: GSM2576294: RNA-Seq AMPK-KO rep2; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 18.7M spots, 1.4G bases, 738.5Mb downloads

Submitted by: NCBI (GEO)

Study: Expression profiling of AMPK-regulated genes in mouse embryonic fibroblast cells

PRJNA382782 • SRP103860 • All experiments • All runs

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We used RNA-Seq assay to characterize AMPK-regulated gene expression patterns and then compared with EZH2-dependent transcriptional profile in MEF. We wanted to determine whether and how AMPK and EZH2 co-regulate a subset of genes. Overall design: AMPKa WT and DKO MEFs were used for RNA-Seq assays in order to compare the transcriptional profiles that are regulated by either AMPK or EZH2.

Sample: RNA-Seq AMPK-KO rep2

SAMN06718677 • SRS2122384 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was extracted using the RNeasy mini kit (Qiagen, Cat#74104) according to the manufacturer's instructions. 1 ug of total RNA was used to construct the libraries using Illumina True-seq RNA sample preparation kit (RS-122-2001). Samples were sequenced using Illumina HiSeq2000 platform.

Experiment attributes:

GEO Accession: GSM2576294

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 18.7M spots, 1.4G bases, 738.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR5445511	18,735,099	1.4G	738.5Mb	2017-12-17

ID:: 3938722

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