GSM2584753: PE RNA rep2; Homo sapiens; RNA-Seq - SRA

SRX2747194: GSM2584753: PE RNA rep2; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 43.6M spots, 2.2G bases, 972.2Mb downloads

Submitted by: NCBI (GEO)

Study: 5hmC dynamically correlated with enhancer's activities during hES-to-Pancreatic endoderm cell differentiation (RNA-Seq)

PRJNA383582 • SRP104304 • All experiments • All runs

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We generated 5hmC, WGBS and RNAseq of the cells in 5 stages during hES-to-pancreatic endoderm cell differantiation, and investigated the 5hmC correlation with 4 histones (H3K4me1, H3K4me3, H3K27ac and H3K27me3) during this process. Our results showed that 5hmC is dynamically correlate with enhancers' activities. Overall design: Purpose: Determine the roles of 5hmC during hES to pancreatic endoderm cell differentiation process. The whole genome bisulfite sequencing, CMS-IP, ATAC-seq and RNAseq for the 5 stages (ES,DE,GT,FG,PE) have been sequenced.

Sample: PE RNA rep2

SAMN06767333 • SRS2133497 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was isolated using Qiagen RNeasy mini kit. RNA library was constructed according to illumina Trueseq kit protocol

Experiment attributes:

GEO Accession: GSM2584753

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 43.6M spots, 2.2G bases, 972.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR5459181	43,563,618	2.2G	972.2Mb	2018-04-19

ID:: 3956072

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