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SRX2792364: GSM2610015: dCas9-1_5; Homo sapiens; OTHER
1 ILLUMINA (Illumina HiSeq 2500) run: 5.5M spots, 552.5M bases, 183.9Mb downloads

Submitted by: NCBI (GEO)
Study: Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor
show Abstracthide Abstract
Even the latest generation of base editor (BE3) causes unwanted indels and non-C-to-T substitutions, compromising the fidelity of base editing outcome. Here we report a enhanced base editing system. The enhanced base edting system decreased the formation of unintended indels and C-to-A/C-to-G substitutions, and increased the frequency of desired C-to-T substitution, thereby improving both the accuracy and efficiency of base editing. Overall design: Examination of indels and base substitutions induced by the enhanced base editing system
Sample: dCas9-1_5
SAMN06920503 • SRS2173824 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2500
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: SINGLE
Construction protocol: For DNA, 72 hr after transfection, the genomic DNA was extracted from the cells with QuickExtractTM DNA Extraction Solution (QE09050, Epicentre). For DNA, DNA-seq libraries were prepared with Illumina TruSeq ChIP Sample Preparation Kit. Deep sequencing was performed on Illumina Hiseq 2500 and Hiseq X ten at CAS-MPG Partner Institute for Computational Biology Omics Core, Shanghai, China.
Experiment attributes:
GEO Accession: GSM2610015
Links:
Runs: 1 run, 5.5M spots, 552.5M bases, 183.9Mb
Run# of Spots# of BasesSizePublished
SRR55189755,524,936552.5M183.9Mb2017-08-29

ID:
4026399

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