Instrument: Ion Torrent Proton
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Tumor tissue was snap frozen w hours after the 12th dose of treatment. Total RNA was extracted using the Macherey Nagel RNA Plus kit cDNA libraries were constructed using the Ion AmpliSeq AmpliSe DNA and RNA Library kit with custome primers(Thermo Fisher) An Ion AmpliSeq™ Custom Panel was designed by the manufacturer (thermo Fisher) using Ion AmpliSeq™ Designer for targeted amplification of 3826 mouse genes , A short amplicon (~110 bp) is amplified for each target