GSM2748331: Mkx_KO_H3K27ac; Mus musculus; ChIP-Seq - SRA

SRX3111004: GSM2748331: Mkx_KO_H3K27ac; Mus musculus; ChIP-Seq
6 ILLUMINA (Illumina HiSeq 2500) runs: 19.4M spots, 970.1M bases, 430.2Mb downloads

Submitted by: NCBI (GEO)

Study: Epigenetic Analysis of Wild Type and Mkx-/- Tendon by Next Generation Sequencing

PRJNA399480 • SRP115933 • All experiments • All runs

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We investigaed the epigenetic landscape of H3K27ac modification between wild type(WT) and Mkx-/- tendon. Overall design: ChIP-seq analsis of H3K27ac modification of tail tendon derived cells from 3-week old wild type (WT) and Mkx-/- mice were performed according to standard protocol in triplicate.

Sample: Mkx_KO_H3K27ac

SAMN07533400 • SRS2446086 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with H3K27ac antibody. Libraries were constructed using NEBNext Ultra II DNA Library Prep Kit(E7645) following manufacture instructions.

Experiment attributes:

GEO Accession: GSM2748331

Links:

NCBI link: NCBI Entrez (gds)

Runs: 6 runs, 19.4M spots, 970.1M bases, 430.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR5952620	4,000,000	200M	88.6Mb	2020-07-01
SRR5952621	4,000,000	200M	88.1Mb	2020-07-01
SRR5952622	4,000,000	200M	89.2Mb	2020-07-01
SRR5952623	4,000,000	200M	88.4Mb	2020-07-01
SRR5952624	1,591,274	79.6M	35.5Mb	2020-07-01
SRR5952625	1,810,563	90.5M	40.3Mb	2020-07-01

ID:: 4404932

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