GSM2748807: TTC_SC_413; Mus musculus; RNA-Seq - SRA

SRX3111424: GSM2748807: TTC_SC_413; Mus musculus; RNA-Seq
1 ILLUMINA (HiSeq X Ten) run: 132,463 spots, 39.7M bases, 17.2Mb downloads

Submitted by: NCBI (GEO)

Study: Divergent cell differentiation trajectory in normal and heterotopic ossified soft connective tissues

PRJNA399478 • SRP115935 • All experiments • All runs

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We used single-cell RNA sequencing and a gene knockout mice model to reveal the heterogeneity and differentiation trajectory of tendon derived cells in both physiological and pathoplogical conditons. Overall design: Wild type(WT) and Mkx-/- tail tendon cells derived from 4-week-old mice were captured on a high-throughput IFC microfluidic Fluidigm C1 system. Wells with no cell or more than one cells were excluded for further analysis, resulting in 227 WT tail tendon cells and 224 Mkx-/- tail tendon cells.

Sample: TTC_SC_413

SAMN07533770 • SRS2446503 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: HiSeq X Ten

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Single-cell capture, RNA extraction and cDNA synthesis were performed following the methods described in the Fluidigm protocol(PN 100-9886) The cDNA reaction products were quantified using the Qubit and were then diluted to a final concentration of 0.2 ng/ μl using C1 Harvest Reagent. The diluted cDNA reaction products were then converted into mRNA-seq libraries using the Nextera XT DNA Sample Preparation Kit (Illumina, FC-131-1096, -2001 and -2002) following the manufacturer’s instructions.

Experiment attributes:

GEO Accession: GSM2748807

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 132,463 spots, 39.7M bases, 17.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR5953050	132,463	39.7M	17.2Mb	2020-07-01

ID:: 4405352

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