GSM1189076: On_Chip_Stimulation_LPS_4h_S38; Mus musculus; RNA-Seq - SRA

SRX323853: GSM1189076: On_Chip_Stimulation_LPS_4h_S38; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 404,820 spots, 101.2M bases, 58.6Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Single-cell RNA-seq reveals dynamic paracrine control of cellular variation

PRJNA212439 • SRP027537 • All experiments • All runs

show Abstracthide Abstract

Here, we use a microfluidics-based approach to prepare single-cell RNA-Seq libraries from over 1,700 primary mouse dendritic cells (DCs) stimulated with three pathogenic components and examine variation between individual cells exposed to the same stimulus and general strategies that multicellular populations use to establish complex dynamic responses. Overall design: RNA seq libraries from 1775 single cells and population controls with SMARTer

Sample: On_Chip_Stimulation_LPS_4h_S38

SAMN02257715 • SRS460218 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: cDNA from each sample was prepared using the SMARTer cDNA synthesis (Clontech). RNA libraries were prepared for sequencing using Nextera XT (Illumina).

Experiment attributes:

GEO Accession: GSM1189076

Links:

External link: GEO Sample

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 404,820 spots, 101.2M bases, 58.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR935940	404,820	101.2M	58.6Mb	2014-06-11

ID:: 452490

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