Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA) from magnetic bead affinity-enriched CD34+ cells (>90%) obtained from marrow aspirates (Miltenyi Biotec, Auburn, CA). The samples were snap frozen in liquid nitrogen and stored at -80oC on the day of collection. Quality check was performed by Bioanalyzer2100 (Agilent Technologies, Palo Alto, CA) using High Sensitivity RNA Chips. 5-10ng of total RNA (RIN>7) was amplified by using the SMARTer Ultra Low RNA Kit for Illumina Sequencing (Clontech Laboratories, Inc., Mountain View, CA) after testing for the fidelity of the protocol on HeLa cell derived RNA (Supplementary Methods, Suppl Figure 1 of publication). cDNA library synthesis and sequencing were performed as previously described (See Supplementary Methods of publication). The libraries were sequenced on the Illumina HiSeq 2000 platform at the Stanford Sequencing Center.