Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted using mirVanaTM miRNA isolation kit (Ambion) according to the manufacturer’s protocol. RNAseq analysis was performed in Harvard genetic facility. Ribosomal reduction using Qiagen kit gene read rRNA depletion kit - http://www.qiagen.com/Products/Catalog/Assay-Technologies/Next-Generation-Sequencing/GeneRead-rRNA-Depletion-Kit#orderinginformation. Direction mRNA sequencing Integenix protocol - http://www.genome.med.harvard.edu/documents/illumina/IntegenX_Apollo324_mRNA_Seq_Protocol_10012012.pdf RNA libraries were prepared for sequencing using standard Illumina protocols