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SRX4037333: GSM3130810: LPS_IC_DMSO_REP2_002; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 16.7M spots, 2.2G bases, 977.7Mb downloads

Submitted by: NCBI (GEO)
Study: Effect of Notch Signaling Inhibitor on Gene Expression in Lipopolysaccharide-stimulated Macrophages in the Presence of Immune Complex
show Abstracthide Abstract
Lipopolysaccharide (LPS)/immunue complex (IC) stimulated macrophages produce cytokine profiles that differe from LPS-stimulated inflammatory macropahges. Notch signaling is activated in LPS/IC-stimulated macrophages and inhibition of Notch signaling reduced IL-10 production. This study investigated the effect of gamma-secretase inhibitor (GSI) that suppresses Notch signaling pathway, on gene expression profiles in macrophages activated by LPS/IC. Overall design: Gamma secretase inhibitor (GSI)-treated bone marrow derived macrophage mRNA profiles were generated by deep sequencing using Illumina NextSeq 500. The results were compared between vehicle control (dimethylsulfoxide)-treated cells and GSI-treated cells.
Sample: LPS_IC_DMSO_REP2_002
SAMN09060765 • SRS3255048 • All experiments • All runs
Organism: Mus musculus
Instrument: NextSeq 500
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA samples were extracted from LPS/IC-activated BMDMs in the presence of DMSO or GSI for 1 hr using an RNeasy Mini Kit (Qiagen, USA). RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer (Agilent, USA) and quantified using a Qubit 3.0 fluorometer (Life Technologies, USA). RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM3130810
Runs: 1 run, 16.7M spots, 2.2G bases, 977.7Mb
Run# of Spots# of BasesSizePublished


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